Journal: Frontiers in Immunology

Article Title: Comprehensive characterization of SLC41A3 identifies it as an immune-related prognostic biomarker and therapeutic target in hepatocellular carcinoma

doi: 10.3389/fimmu.2026.1861310

Figure Lengend Snippet: SLC41A3 promotes HCC progression. (A) Western blot analysis confirming the knockdown efficiency of SLC41A3 in Hep3B and HuH7 cells transfected with shRNA targeting SLC41A3. (B) CCK-8 assay evaluating cell proliferation at specified time points after SLC41A3 silencing in HCC cells. (C) Colony formation ability of Hep3B and HuH7 cells was inhibited after SLC41A3 knockdown. (D) Transwell migration and invasion assays indicated impaired migration and invasion capabilities after SLC41A3 downregulation in both cell lines. (E) Wound healing assay showed reduced cell motility after SLC41A3 knockdown. Scale bar, 100 μm. Data are presented as the mean ± SD of three independent experiments. *** P < 0.001.

Article Snippet: The human HCC cell lines Hep3B and Huh7 were purchased from Procell Biotechnology.

Techniques: Western Blot, Knockdown, Transfection, shRNA, CCK-8 Assay, Migration, Wound Healing Assay

Journal: bioRxiv

Article Title: Light-dependent cell fixing with DNA-targeting fluorophores

doi: 10.64898/2026.03.27.714905

Figure Lengend Snippet: (A) Localization of PAL (green) at mtDNA within MitoRed ® -stained mitochondria (red) in live Hep3B cells prior to irradiation (scale bar: 10 µm, crop: 5 µm). (B) Top: snapshots of time-lapse PAL nuclear fluorescence upon WF irradiation (scale bar: 20 µm). Bottom: PAL labeling upon irradiation at distinct phases of the cell cycle ( a : interphase; b : prophase; c : prometaphase; d : metaphase; e : anaphase. Scale bar: 5 µm). Right : kinetics of PAL fluorogenesis during 5 min irradiation. Mean intensities are rescaled from 0 to 1 within a 95% confidence interval. (C) PAL nuclear fluorescence (in green) of a targeted cell islet within a population of live PAL-treated cells upon WF irradiation (PAL + hv) (R1: dotted line; R2: dashed line; NA: non-affected area. Scale bar: 50 µm). Crops in R1 (orange squares) showing stable nuclear morphology and persistence up to 9 days post-irradiation within a live-cell proliferative environment. Crops in R2 (blue squares) showing compromised nuclei. Calculated irradiation powers were 3.5 to 15 W/cm 2 in R1 from limit to center of the beam. Only evanescent or residual scattered light was present in R2 (scale bar: 20 µm). (D) Co-staining of PAL-treated cells (green) with propidium iodide (PI, red) or NucView ® 530 Red Caspase-3 dye (NucView, magenta). Top: PI stains R1 cells (dotted lines), showing a permeabilized status immediately post-irradiation (d0). After 24 h, R2 cells show important delayed PI staining demonstrating a compromised state. Bottom: NucView faintly stains R1 cells 24 h post-irradiation, showing strong signal in compromised R2 cells. The apoptosis inducer staurosporine is used as a positive control. (E) Quantification of nuclear size in different conditions: live PAL-treated cells without irradiation (PAL), FA-fixed cells (FA), PAL-free irradiated cells (hv) or PAL-treated irradiated cells (PAL + hv, R1 and R2) (n>200 per condition). Data shown in violin plots reflecting size distribution, circles indicate the medians and whiskers indicate standard deviations.

Article Snippet: The human cell line Hep3B was obtained from ATCC ® (HB-8064), the human cell lines T24, PC3 and C4-2B were a courtesy of Pr.

Techniques: Staining, Irradiation, Fluorescence, Labeling, Positive Control

Journal: bioRxiv

Article Title: Light-dependent cell fixing with DNA-targeting fluorophores

doi: 10.64898/2026.03.27.714905

Figure Lengend Snippet: (A) Left: Optofixing procedure of a whole Hep3B cell population using a portable LED lamp towards biochemical analysis. Top right: Visualization of abundance of precipitates (UVA irradiation). Right bottom: Corresponding SDS-PAGE analysis of soluble protein contents following treatments with formaldehyde (FA), 4-hydroxynonenal (4HNE), acrolein (ACR) or LED irradiation in presence or absence of PAL. (B) Left panel: WF imaging of PAL nuclear fluorogenesis at day 0 after irradiation by a WF microscope. Right panel: stability of fixed state after 3 days of PAL-treated cells in presence of inhibitors (scale bar: 10µm). (C) Left: fluorogenesis kinetics during 5 min irradiation with conditions described in B . Fluorescence intensities were normalized as [(I-Io)/Io]. Right : quantification of nuclear sizes with conditions described in B (n>60 per condition) 3 days post-irradiation. ( D) Lipid peroxidation (LPO)-quantification in PAL-treated cells using BODIPY™ 581/591-C11 probe. Top left: representative image of probe staining 30 min after irradiation of PAL-treated cells in zones NA, R1 and R2. Top right: plot of ratiometric measurements along the yellow dashed line. Red curve represents Red/Ox ratio, green curve represents the Ox/Red ratio. Bottom: quantification of the oxidation ratio (Ox/Red) 30 min after irradiation of PAL-treated cells (R1, R2) compared to irradiated cells in absence of PAL (hv) or cells treated with PAL without irradiation (PAL) (n>100 per condition). (E) ROS quantification in PAL-treated cells using ROS Brite TM 670 probe. Top: representative image of ROS staining (in magenta) obtained after irradiation of PAL-treated cells (in green) in R1 and R2 (scale bar: 10 µm). Bottom: Quantification of ROS Brite fluorescence intensity in the same conditions than D .

Article Snippet: The human cell line Hep3B was obtained from ATCC ® (HB-8064), the human cell lines T24, PC3 and C4-2B were a courtesy of Pr.

Techniques: Irradiation, SDS Page, Imaging, Microscopy, Fluorescence, Staining

Journal: bioRxiv

Article Title: Light-dependent cell fixing with DNA-targeting fluorophores

doi: 10.64898/2026.03.27.714905

Figure Lengend Snippet:

Article Snippet: The human cell line Hep3B was obtained from ATCC ® (HB-8064), the human cell lines T24, PC3 and C4-2B were a courtesy of Pr.

Techniques: Labeling

Journal: Cell Death Discovery

Article Title: ATGL sensitizes hepatocellular carcinoma cells to genotoxic drugs by modulating p53 acetylation/phosphorylation status

doi: 10.1038/s41420-026-03048-4

Figure Lengend Snippet: A , B HUH7 and C , D Hep3B cells were transfected with an empty vector (Vehicle) or ATGL-overexpressing construct (ATGL-OE) for 24 h and then treated with 50 µM etoposide or 2 µM doxorubicin for 6 h. Western blot analysis of γH2AX levels was performed. E Western blot analysis of Ac-p53 Lys-382, p-p53 Ser-15 and p53 levels was performed in HepG2 cells after treatment with 50 µM etoposide for 2, 4, 6 and 24 h. F Densitometric ratios of Ac-p53 and p-p53 after treatment with 50 µM etoposide for 2, 4, 6 and 24 h. G – I HepG2 cells were transfected with empty vector (Vehicle) or ATGL-overexpressing (ATGL-OE) construct and, after 24 h, treated with 50 µM etoposide for 6 h with or without 10 µM C646 for 24 h. Western blot analysis of Ac-p53 Lys-382, p-p53 Ser-15 and p53 levels was performed. H Densitometric analysis ratios of Ac-p53 and p-p53 after treatment with 50 µM etoposide for 6 h. J – L HepG2 cells were treated with 50 µM etoposide for 6 h with or without 1 µM GW7647 for 24 h. Western blot analysis of Ac-p53 Lys-382, p-p53 Ser-15, p53 and γH2AX levels was performed. K Densitometric analysis of ratio between Ac-p53 and p-p53 expression after treatment with 50 µM etoposide for 6 h. The images are representative of three independent experiments that yielded similar results. β-Actin and ATGL were used as loading and transfection controls, respectively. The data are presented as the means ± SDs from three independent experiments. Statistical significance was determined by Student t test and one-way ANOVA with Tukey’s post hoc test; * p < 0.05, ** p < 0.01, *** p < 0.001 vs CTRL or as indicated by brackets.

Article Snippet: HepG2, HUH7 and Hep3B cell lines were purchased from American Type Culture Collection (ATCC).

Techniques: Transfection, Plasmid Preparation, Construct, Western Blot, Expressing